DSMZ 827 . THERMOANAEROBACTER SULFUROPHILUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
* Modified for Petrimonas sulfuriphila DSM 16547

Supplement medium with 3.00 g/l Trypticase peptone.

Equipment needed: Hungate tubes Filter Gassing station Autoclave Water bath
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.329 6.157
KCl 0.33 g 0.329 4.418
KH2PO4 0.33 g 0.329 2.42
MgCl2 x 6 H2O 0.33 g 0.329 1.62
CaCl2 x 2 H2O 0.33 g 0.329 2.24
Trace element solution SL-10 1.00 ml - -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 0.002
Sulfur
(powdered)
10.00 g 9.98 311.195
Na2CO3 1.00 g 0.998 9.416
Yeast extract 0.50 g 0.499 -
D-Glucose 5.00 g 4.99 27.698
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.50 g 0.499 2.078
Trypticase peptone* 3.00 g - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients except sulfur, carbonate, yeast extract, glucose, vitamins, and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur. Heat the medium vials to 90-100°C for 1 hour on each of 3 successive days to sterilize the medium. Add yeast extract, glucose, vitamins (sterilized by filtration), and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use check pH of complete medium and adjust to 6.8 - 7.0, if necessary.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 299 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 13.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8 - 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes, Water bath
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 1
Petrimonas sulfuriphila DSM 16547

  3 g Trypticase peptone

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Sulfur 9.98 311.195
D-Glucose 4.99 27.698
Trypticase peptone 2.994 -
Na2CO3 0.998 9.416
Na2S x 9 H2O 0.499 2.078
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 1 strain
phyla 2 2 strains
oxygen anaerobe 2 strains
temperature thermophilic 2 strains
temperature mesophilic 1 strain