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DSMZ

DSMZ 828 . NEVSKIA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Nevskia ramosa DSM 11500

This strain uses the standard medium without modifications.

Growth conditions: 28 °C

Main sol. 828 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Potassium phosphate buffer , 1M, pH 7 27.50 ml 64.35 -
MgSO4 x 7 H2O 0.05 g 0.05 0.203
CaCl2 x 2 H2O 14.00 mg 0.014 0.095
Na-lactate 0.56 g 0.56 4.997
SL-9 0.50 ml - -
Seven vitamins solution 0.50 ml - -
Agar , if necessary 15.00 g 15 44.599
Distilled water 972.50 ml - -
1 Adjust the pH to 7.0 - 7.2.
2 Filter sterilize the vitamin solution and add to the autoclaved medium.

SL-9 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 2.0 g 2 10.06
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Nitrilotriacetic acid 12.8 g 12.8 66.967
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
2 Adjust pH to 6.0 .

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 06.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 2
Strains with modifications: 1
Nevskia ramosa DSM 11500

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Potassium phosphate buffer 64.35 -
Agar 15 44.599
Na-lactate 0.56 4.997
MgSO4 x 7 H2O 0.05 0.203
CaCl2 x 2 H2O 0.014 0.095
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
temperature mesophilic 2 strains