828 .
NEVSKIA MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
7.0 - 7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Potassium phosphate buffer , 1M, pH 7 | 27.50 | ml | ||
|
|
MgSO4 x 7 H2O | 0.05 | g | ||
|
|
CaCl2 x 2 H2O | 14.00 | mg | ||
|
|
Na-lactate | 0.56 | g | ||
|
|
SL-9 | 0.50 | ml | ||
|
|
Seven vitamins solution | 0.50 | ml | ||
|
|
Agar , if necessary | 15.00 | g | ||
|
|
Distilled water | 972.50 | ml | ||
| 1 Adjust the pH to 7.0 - 7.2. | |||||
| 2 Filter sterilize the vitamin solution and add to the autoclaved medium. | |||||
SL-9 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 2.0 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Nitrilotriacetic acid | 12.8 | g | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
| 2 Adjust pH to 6.0 . | |||||
Seven vitamins solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Vitamin B12 | 100 | mg |
|
|
p-Aminobenzoic acid | 80 | mg |
|
|
D-(+)-biotin | 20 | mg |
|
|
Nicotinic acid | 200 | mg |
|
|
Calcium pantothenate | 100 | mg |
|
|
Pyridoxine hydrochloride | 300 | mg |
|
|
Thiamine-HCl x 2 H2O | 200 | mg |
|
|
Distilled water | 1000 | ml |