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DSMZ

DSMZ 829a . DESULFUROBACTERIUM ATLANTICUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 829a #

ml
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 29.412 503.281
MgCl2 x 6 H2O 3.00 g 2.941 14.467
CaCl2 x 2 H2O 0.15 g 0.147 1
KCl 0.50 g 0.49 6.575
NH4Cl 0.50 g 0.49 9.164
MES [2-(N-morpholino) ethane sulfonic acid] 1.95 g 1.912 9.792
KH2PO4 0.20 g 0.196 1.441
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
Na2S2O3 x 5 H2O 2.50 g 2.451 9.876
Neutralized sulfide solution 3% (w/v) 20.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except thiosulfate and sodium sulfide), adjust pH to 6.0, boil medium for 1 min, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add sodium thiosulfate from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Reduce medium with a sterile, neutralized solution of sodium sulfide prepared under 100% N2 gas . Adjust pH of complete medium to 6.0, if necessary.
2 After inoculation pressurize cultivation vessels to 2 bar overpressure using sterile 80% H2 and 20% CO2 gas mixture.

Neutralized sulfide solution 3% (w/v) #

100
Solution occurs in 18 other media (first defined in Medium 28).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 3 g 30 124.905
Distilled water 100 ml - -
1 The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121°C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle.

Metadata

Last modified: 27.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 29.412 503.281
MgCl2 x 6 H2O 2.941 14.467
Na2S2O3 x 5 H2O 2.451 9.876
MES [2-(N-morpholino) ethane sulfonic acid] 1.912 9.792
Na2S x 9 H2O 0.588 2.449
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain