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DSMZ

DSMZ 829b . THERMODESULFOBACTERIUM HYDROGENIPHILUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 829b #

ml
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sea Salt
(SIGMA) 		30.00 g 29.851 -
NH4Cl 1.00 g 0.995 18.602
KH2PO4 0.35 g 0.348 2.559
MES [2-(N-morpholino) ethane sulfonic acid] 1.95 g 1.94 9.938
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Na2CO3 0.50 g 0.498 4.694
Yeast extract 0.50 g 0.498 -
Seven vitamins solution 1.00 ml - -
Growth-stimulating factors 1.00 ml - -
Na-dithionite solution (5% w/v) 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, yeast extract, vitamins, growth-stimulating factors and Na-dithionite), boil medium for 1 min, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere. Adjust pH to 6.0 and dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add yeast extract, vitamins, and growth-stimulating factors from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and carbonate and Na-dithionite from sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins and Na-dithionite are sterilized by filtration. Adjust pH of the complete medium to 6.5, if necessary.
2 After inoculation use 2 bar overpressure of sterile 80% H2 and 20% CO2 gas mixture.

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 178 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Growth-stimulating factors #

1000
Solution occurs in 1 other media (first defined in Medium 829).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Isobutyric acid 5 g 5 56.747
Valeric acid 5 g 5 48.957
2-Methylbutyric acid 5 g 5 48.957
3-Methylbutyric acid 5 g 5 48.956
Caproic acid 2 g 2 17.218
Succinic acid 6 g 6 50.809
Distilled water 1000 ml - -
1 Dissolve ingredients, adjust pH to 9.0 with NaOH, then autoclave under 100% N2 gas.

Na-dithionite solution (5% w/v) #

1000
Solution occurs in 7 other media (first defined in Medium 829).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 50 g 50 595.188
Na2S2O4 50 g 50 287.175
Distilled water 1000 ml - -
1 Dissolve NaHCO3 in water and make the solution anoxic by sparging with 80% N2 and 20% CO2 gas mixture. Then dissolve the Na-dithionite and filter sterilize the solution into anoxic Hungate tubes. Store the prepared solution in the dark and refrigerated. Prepare only small amounts of stock solution, as Na-dithionite decomposes rapidly.

Metadata

Last modified: 27.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sea Salt 29.851 -
MES [2-(N-morpholino) ethane sulfonic acid] 1.94 9.938
NH4Cl 0.995 18.602
Na2CO3 0.498 4.694
Yeast extract 0.498 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain