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830. R2A MEDIUM

Culture medium recipe

Vol.
×
Solution occurs in 2 other media (first defined in medium 830 )
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.50 g 0.5 -
2
(Difco no. 3)
0.50 g 0.5 -
3 0.50 g 0.5 -
4 0.50 g 0.5 2.775
5 0.50 g 0.5 -
6 0.30 g 0.3 2.726
7 0.30 g 0.3 1.722
8 0.05 g 0.05 0.203
9 , adjust if required 15.00 g 15 -
10 1000.00 ml 998.207 55408.8
Instructions:
  1. Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave

Associated strains

All strains for this medium: 1548
Strains with modifications: 209
Caballeronia mineralivorans DSM 104028

No modifications necessary for this strain.

Light

215 strains need light for growth. Select a strain for more details.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Agar 15 -
Glucose 0.5 2.775
Yeast extract 0.5 -
Proteose peptone 0.5 -
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Cultivation metadata from 1548 strains BacDive

halophily not 405 strains
halophily slightly 193 strains
halophily moderate 95 strains
halophily extreme 5 strains
pH 6 - 8 487 strains
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Similar media

830c. REACTIVATION WITH LIQUID MEDIUM 830 100 %
919. LIMNOBACTER MEDIUM 100 %
1492. HALF STRENGTH R2A 99 %
1641. DOUBLE CONCENTRATED 830 + 0,06% SODIUM PYRUVATE 99 %
1578. 1/10 R2A MEDIUM 99 %

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