DSMZ 830b . MEDIUM R2A FOR DENITRIFYING BACTERIA

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.50 g 0.5 -
Proteose peptone
(Difco no. 3)
0.50 g 0.5 -
Casamino acids 0.50 g 0.5 -
Glucose 0.50 g 0.5 2.775
Starch
(soluble)
0.50 g 0.5 -
Na-pyruvate 0.30 g 0.3 2.726
K2HPO4 0.30 g 0.3 1.722
MgSO4 x 7 H2O 0.05 g 0.05 0.203
KNO3 0.50 g 0.5 4.945
Trace element solution SL-10 10.00 ml - -
Distilled water 1000.00 ml - -
1 Prepare liquid medium under anaerobic conditions (N2).
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 20.12.21
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KNO3 0.5 4.945
Glucose 0.5 2.775
Yeast extract 0.5 -
Proteose peptone 0.5 -
Casamino acids 0.5 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen aerobe 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain