Name: MEDIUM R2A FOR DENITRIFYING BACTERIA
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 830b #

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Yeast extract	0.50	g	0.5	-
Proteose peptone (Difco no. 3)	0.50	g	0.5	-
Casamino acids	0.50	g	0.5	-
Glucose	0.50	g	0.5	2.775
Starch (soluble)	0.50	g	0.5	-
Na-pyruvate	0.30	g	0.3	2.726
K₂HPO₄	0.30	g	0.3	1.722
MgSO₄ x 7 H₂O	0.05	g	0.05	0.203
KNO₃	0.50	g	0.5	4.945
Trace element solution SL-10	10.00	ml	-	-
Distilled water	1000.00	ml	-	-
1 Prepare liquid medium under anaerobic conditions (N₂).

Trace element solution SL-10 #

1000

Solution occurs in 265 other media (first defined in Medium 320).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%)	10.0	ml	2.5	68.568
FeCl₂ x 4 H₂O	1.5	g	1.5	7.545
ZnCl₂	70.0	mg	0.07	0.514
MnCl₂ x 4 H₂O	100.0	mg	0.1	0.505
H₃BO₃	6.0	mg	0.006	0.097
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	990.0	ml	-	-
1 First dissolve FeCl₂ in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

All strains for this medium:

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830	R2A MEDIUM
a	MEDIUM R2A MODIFIED
b	MEDIUM R2A FOR DENITRIFYING BACTERIA
c	REACTIVATION WITH LIQUID MEDIUM 830
d	R2A MEDIUM (MICROAEROBIC)