DSMZ 830c . REACTIVATION WITH LIQUID MEDIUM 830

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Selected strain: Xanthomonas arboricola DSM 18808

This strain uses the standard medium without modifications.

Compound Amount Unit Conc. [g/L] Conc. [mM]
830. R2A MEDIUM 1000 ml - -
1 Rehydrate and grow lyophilized cells from ampoules in liquid broth 830. Subsequent subculturing may be carried out in liquid medium or on agar plates.
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.50 g 0.5 -
Proteose peptone
(Difco no. 3)
0.50 g 0.5 -
Casamino acids 0.50 g 0.5 -
Glucose 0.50 g 0.5 2.775
Starch
(soluble)
0.50 g 0.5 -
Na-pyruvate 0.30 g 0.3 2.726
K2HPO4 0.30 g 0.3 1.722
MgSO4 x 7 H2O 0.05 g 0.05 0.203
Agar 15.00 g 15 44.599
Distilled water 1000.00 ml - -
1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 94
Strains with modifications: 5
Xanthomonas arboricola DSM 18808

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Glucose 0.5 2.775
Yeast extract 0.5 -
Proteose peptone 0.5 -
Casamino acids 0.5 -
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Cultivation metadata from 94 strains BacDive

halophily non-halophilic 1 strain
pH 6 - 8 32 strains
pH 8 - 10 15 strains
pH 4 - 6 11 strains
pH 10 - 12 2 strains
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