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DSMZ

DSMZ 833 . DEHALOSPIRILLUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Sulfurospirillum multivorans DSM 12446

This strain uses the standard medium without modifications.

Growth conditions: anaerobic 25 °C

Main sol. 833 #

ml
Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 892 ml - -
Solution B 10 ml - -
Solution C 2 ml - -
Solution D 35 ml - -
Solution E 20 ml - -
Solution F 40 ml - -
Solution G 3 ml - -
Solution H 1 ml - -
1 Sparge solution A with 100% N2 gas for 30 - 45 min to make it anoxic, then distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B and H are autoclaved separately under 100% N2 gas. Solutions C, E, F and G are prepared under 100% N2 gas and sterilized by filtration. Solution D is autoclaved under 80% N2 and 20% CO2 gas atmosphere. To complete the medium appropriate amounts of solutions B to H are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.3 - 7.6, if necessary.
2 Note: Before inoculation, the medium must be completely reduced, i.e. resazurin must be colorless. The addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized, see medium 829) can help to reduce the medium.

Solution A #

892
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 0.70 g 0.785 5.525
KH2PO4 0.20 g 0.224 1.648
NH4Cl 0.25 g 0.28 5.24
NaCl 0.25 g 0.28 4.796
MgCl2 x 6 H2O 0.40 g 0.448 2.206
KCl 0.50 g 0.561 7.519
CaCl2 x 2 H2O 0.15 g 0.168 1.144
Yeast extract 2.00 g 2.242 -
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.6e-4 0.002
Distilled water 890.00 ml - -

Solution B #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Potassium phosphate buffer
(0.1 M, pH 7.5) 		10 ml - 1

Solution C #

2
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution (10x) 1 ml - -
Seven vitamins solution 1 ml - -

Solution D #

35
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1.75 g 50 471.752
Distilled water 35.00 ml - -

Solution E #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-pyruvate 4.5 g 225 2044.64
Distilled water 20.0 ml - -

Solution F #

40
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-fumarate 6.4 g 160 999.775
Distilled water 40.0 ml - -

Solution G #

7
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 30 mg 4.286 15.415
H2SO4
(0.1 N) 		3 ml - -

Solution H #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Cysteine HCl x H2O 50 mg 50 284.68
Distilled water 1 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 179 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Wolin's vitamin solution (10x) #

1000
Solution occurs in 306 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 26.07.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3 - 7.6
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Sulfurospirillum multivorans DSM 12446

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2-fumarate 6.381 39.871
Na-pyruvate 4.487 40.771
Yeast extract 1.994 -
Na2CO3 1.745 16.462
Na2SO4 0.698 4.913
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains