DSMZ 836 . DESULFITOBACTERIUM FRAPPIERI MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.27 g 0.27 1.984
K2HPO4 0.35 g 0.35 2.009
NH4Cl 0.53 g 0.53 9.908
MgCl2 x 6 H2O 0.10 g 0.1 0.492
FeCl2 x 4 H2O
(0.1% w/v in 0.2 N HCl)
2.00 ml 0.002 0.01
CaCl2 x 2 H2O 0.07 g 0.07 0.476
Trace element solution SL-10 1.00 ml - -
Yeast extract 1.00 g 1 -
Na-pyruvate 6.05 g 6.05 54.978
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
Na2CO3 0.50 g 0.5 4.718
Na2SO3 0.63 g 0.63 4.998
Na2S x 9 H2O 0.05 g 0.05 0.208
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, sulfite and sulfide), adjust pH of solution to 7.0 and sparge with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to use add sulfite and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The stock solution of sulfite should by freshly prepared and sterilized by filtration. Prior to use check pH of complete medium and adjust to 7.5, if necessary.
Solution occurs in 262 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 07.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na-pyruvate 6.05 54.978
Yeast extract 1 -
Na2SO3 0.63 4.998
NH4Cl 0.53 9.908
Na2CO3 0.5 4.718
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen anaerobe 3 strains
temperature mesophilic 3 strains