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836. DESULFITOBACTERIUM FRAPPIERI MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.27 g 0.269 1.976
2 0.35 g 0.349 2.001
3 0.53 g 0.528 9.869
4 0.10 g 0.1 0.49
5
(0.1% w/v in 0.2 N HCl)
2.00 ml 0.002 0.01
6 0.07 g 0.07 0.474
7 Trace element solution SL-10 1.00 ml - -
8 1.00 g 0.996 -
9 6.05 g 6.026 54.759
10
(0.1% w/v)
0.50 ml 5.0e-4 0.002
11 0.50 g 0.498 4.699
12 0.63 g 0.627 4.978
13 0.05 g 0.05 0.207
14 1000.00 ml 994.23 55188.1
15 , might need strain adjustment 3.00 g/l - -
Instructions:
  1. Dissolve ingredients (except carbonate, sulfite and sulfide), adjust pH of solution to 7.0 and sparge with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to use add sulfite and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The stock solution of sulfite should by freshly prepared and sterilized by filtration. Prior to use check pH of complete medium and adjust to 7.5, if necessary.
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 3
Strains with modifications: 3

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 995.214 55242.7
Na-pyruvate 6.026 54.759
Yeast extract 0.996 -
Na2SO3 0.627 4.978
NH4Cl 0.528 9.869
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen anaerobe 3 strains
temperature mesophilic 3 strains

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