836
836 .
DESULFITOBACTERIUM PCP-1 MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Desulfitobacterium hafniense DSM 12420
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
37 °C
Main sol. 836 #
ml
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.27 | g | 0.27 | 1.984 | |
| K2HPO4 | 0.35 | g | 0.35 | 2.009 | |
| NH4Cl | 0.53 | g | 0.53 | 9.908 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.492 | |
|
FeCl2 x 4 H2O
(0.1% w/v in 0.2 N HCl) |
2.00 | ml | 0.002 | 0.01 | |
| CaCl2 x 2 H2O | 0.07 | g | 0.07 | 0.476 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Yeast extract | 1.00 | g | 1 | - | |
| Na-pyruvate | 6.05 | g | 6.05 | 54.978 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Na2CO3 | 0.50 | g | 0.5 | 4.718 | |
| Na2SO3 | 0.63 | g | 0.63 | 4.998 | |
| Na2S x 9 H2O | 0.05 | g | 0.05 | 0.208 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, sulfite and sulfide), adjust pH of solution to 7.0 and sparge with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to use add sulfite and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The stock solution of sulfite should by freshly prepared and sterilized by filtration. Prior to use check pH of complete medium and adjust to 7.5, if necessary. | |||||
Solution occurs in 265
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 07.11.22 |
| Source: | DSMZ |
| Synonyms: | DESULFITOBACTERIUM FRAPPIERI MEDIUM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 3 |
| Strains with modifications: | 1 |
Desulfitobacterium hafniense DSM 12420
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na-pyruvate | 6.05 | 54.978 |
| Yeast extract | 1 | - |
| Na2SO3 | 0.63 | 4.998 |
| NH4Cl | 0.53 | 9.908 |
| Na2CO3 | 0.5 | 4.718 |
| Show more | ||
