Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.27 | g | 0.27 | 1.984 | |
K2HPO4 | 0.35 | g | 0.35 | 2.009 | |
NH4Cl | 0.53 | g | 0.53 | 9.908 | |
MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.492 | |
FeCl2 x 4 H2O
(0.1% w/v in 0.2 N HCl) |
2.00 | ml | 0.002 | 0.01 | |
CaCl2 x 2 H2O | 0.07 | g | 0.07 | 0.476 | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
Yeast extract | 1.00 | g | 1 | - | |
Na-pyruvate | 6.05 | g | 6.05 | 54.978 | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
Na2CO3 | 0.50 | g | 0.5 | 4.718 | |
Na2SO3 | 0.63 | g | 0.63 | 4.998 | |
Na2S x 9 H2O | 0.05 | g | 0.05 | 0.208 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except carbonate, sulfite and sulfide), adjust pH of solution to 7.0 and sparge with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to use add sulfite and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The stock solution of sulfite should by freshly prepared and sterilized by filtration. Prior to use check pH of complete medium and adjust to 7.5, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 990.0 | ml | - | - | |
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. |
Last modified: | 07.11.22 |
Source: | DSMZ |
Synonyms: | DESULFITOBACTERIUM FRAPPIERI MEDIUM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.5 |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Na-pyruvate | 6.05 | 54.978 |
Yeast extract | 1 | - |
Na2SO3 | 0.63 | 4.998 |
NH4Cl | 0.53 | 9.908 |
Na2CO3 | 0.5 | 4.718 |
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