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DSMZ 837 . DESULFUROMONAS PALMITATIS MEDIUM

Culture medium recipe

Vol.
×

Main sol. 837 #

ml
Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 870 ml - -
Solution B 50 ml - -
Solution C 20 ml - -
Solution D 10 ml - -
Solution E 50 ml - -
1 Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 minto make it anoxic, distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 100% N2 gas atmosphere. Solution C is prepared under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 6.9, if necessary. Prior to inoculation medium is reduced by addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized).
2 Note: For transfers use 5 - 10% (v/v) inoculum.

Solution A #

870
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.80 g 23.908 409.104
KCl 0.77 g 0.885 11.872
NH4Cl 1.00 g 1.149 21.489
KH2PO4 0.10 g 0.115 0.845
MgSO4 x 7 H2O 0.20 g 0.23 0.933
CaCl2 x 2 H2O 0.02 g 0.023 0.156
Na-acetate x 3 H2O 1.36 g 1.563 -
Modified Wolin's mineral solution 10.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 -
Distilled water 860.00 ml - -

Solution B #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 10.6 g 212 1042.78
CaCl2 x 2 H2O 1.5 g 30 204.061
Distilled water 50.0 ml - -

Solution C #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1 g 50 471.752
Distilled water 20 ml - -

Solution D #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution 10 ml - -

Solution E #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-fumarate 8 g 160 999.775
Distilled water 50 ml - -

Modified Wolin's mineral solution #

1000
Solution occurs in 189 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Wolin's vitamin solution #

1000
Solution occurs in 351 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.9
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes

Gas composition

  • 20 % CO2
  • 80 % N2

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 10.6 52.139
Na2-fumarate 8 49.989
CaCl2 x 2 H2O 1.5 10.203
Na-acetate x 3 H2O 1.36 -
NH4Cl 1 18.695
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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