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DSMZ

DSMZ 837 . DESULFUROMONAS PALMITATIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 837 #

ml
Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 880 ml - -
Solution B 50 ml - -
Solution C 20 ml - -
Solution D 1 ml - -
Solution E 50 ml - -
Solution F 1 ml - -
1 Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 100% N2 gas atmosphere. Solutions C and F are prepared under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere. Solution C is autoclaved and solutions D, E and F are sterilized by filtration. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 6.9, if necessary.
2 Note: For transfers use 5 - 10% (v/v) inoculum.

Solution A #

870
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.80 g 23.908 409.104
KCl 0.77 g 0.885 11.872
NH4Cl 1.00 g 1.149 21.489
KH2PO4 0.10 g 0.115 0.845
MgSO4 x 7 H2O 0.20 g 0.23 0.933
CaCl2 x 2 H2O 0.02 g 0.023 0.156
Na-acetate x 3 H2O 1.36 g 1.563 -
Modified Wolin's mineral solution 10.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.7e-4 0.002
Distilled water 860.00 ml - -

Solution B #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 10.6 g 212 1042.78
CaCl2 x 2 H2O 1.5 g 30 204.061
Distilled water 50.0 ml - -

Solution C #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1 g 50 471.752
Distilled water 20 ml - -

Solution D #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution (10x) 1 ml - -

Solution E #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-fumarate 8 g 160 999.775
Distilled water 50 ml - -

Solution F #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-dithionite solution (5% w/v) 1 ml - -

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Na-dithionite solution (5% w/v) #

1000
Solution occurs in 7 other media (first defined in Medium 829).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 50 g 50 595.188
Na2S2O4 50 g 50 287.175
Distilled water 1000 ml - -
1 Dissolve NaHCO3 in water and make the solution anoxic by sparging with 80% N2 and 20% CO2 gas mixture. Then dissolve the Na-dithionite and filter sterilize the solution into anoxic Hungate tubes. Store the prepared solution in the dark and refrigerated. Prepare only small amounts of stock solution, as Na-dithionite decomposes rapidly.

Metadata

Last modified: 13.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.9
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 10.579 52.035
Na2-fumarate 7.984 49.889
CaCl2 x 2 H2O 1.497 10.183
Na-acetate x 3 H2O 1.373 -
NH4Cl 1.009 18.872
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain