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DSMZ

DSMZ 838 . GEOANAEROBACTER MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Desulfitobacterium hafniense DSM 16228

* Modification: Omit Na-acetate and Na-ascorbate. Supplement medium after autoclaving with 10.00 ml/l of a Wolin's vitamin solution (see medium 141), 1.00 g/l Na-DL-lactate and 1.00 g/l yeast extract added from sterile anoxic stock solutions prepared under 100% N2 gas.

Growth conditions: anaerobic 30 °C

Main sol. 838 #

ml
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ferric citrate monohydrate 10.0 g 9.98 37.953
KH2PO4 0.6 g 0.599 4.4
NH4Cl 0.3 g 0.299 5.597
MgSO4 x 7 H2O 0.5 g 0.499 2.025
CaCl2 x 2 H2O 0.1 g 0.1 0.679
Trace element solution SL-11 1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
Na2CO3 1.5 g 1.497 14.124
Na-acetate* 0.8 g 0.798 9.733
Na-ascorbate* 0.8 g 0.798 4.03
Na-(DL)-lactate* 1.0 g - -
Yeast extract* 1.0 g - -
Wolin's vitamin solution* 10.0 ml - -
Distilled water 1000.0 ml - -
1 First dissolve ferric citrate by heating the water under continuous stirring. After cooling to room temperature adjust the pH to 6.0, then add and dissolve the remaining ingredients, except carbonate, acetate and ascorbate. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add acetate and ascorbate from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to inoculation check the medium pH and adjust to 6.8, if necessary.

Trace element solution SL-11 #

1000
Solution occurs in 22 other media (first defined in Medium 722).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA x 2 H2O 5.2 g 5.2 15.467
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -
1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.

Selenite-tungstate solution #

1000
Solution occurs in 178 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Wolin's vitamin solution * #

1000
Solution occurs in 53 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 23.05.23
Source: DSMZ
Synonyms: GEOBACTER PELOPHILUS MEDIUM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 9
Strains with modifications: 8
Desulfitobacterium hafniense DSM 16228

  1 g Na-(DL)-lactate
  1 g Yeast extract
  Na-acetate
  Na-ascorbate
+  10 ml Wolin's vitamin solution

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Ferric citrate monohydrate 9.98 37.953
Na2CO3 1.497 14.124
Yeast extract 0.998 -
Na-(DL)-lactate 0.998 -
KH2PO4 0.599 4.4
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Cultivation metadata from 9 strains BacDive

pH 6 - 8 3 strains
phyla 3 9 strains
oxygen anaerobe 8 strains
temperature mesophilic 7 strains
temperature psychrophilic 1 strain