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DSMZ

DSMZ 840 . SERPULINA MURDOCHII MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Liquid medium (in 5ml tube) #

ml
Equipment needed: Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase soy broth
(BBL 11774) 		5.00 ml - -
1 Prepare Trypticase Soy Broth (BBL 11774) under 80% N2 + 20% CO2 gas mixture in Hungate tubes (5 ml per tube).
2 After autoclaving and cooling add (per tube):
NaHCO3
(10% w/v; sterile) 		0.30 ml 0.03 0.357
Bovine serum
(sterile; heat inactivated at 56°C for 45 min) 		0.35 ml - -
Glucose
(30% w/v; sterile) 		0.05 ml 0.015 0.083
Air 0.50 ml - -
3 Incubate inoculated tubes in slanted position.

Liquid medium (in 100 ml serum bottle) #

10
Equipment needed: Serum bottle
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase soy broth
(BBL 11774) 		10.0 ml - -
1 Prepare Trypticase Soy Broth (BBL 11774) under 80% N2 + 20% CO2 gas mixture in 100 ml serum bottles (10 ml per bottle).
2 After autoclaving and cooling add per bottle:
Glucose
(30% w/v; sterile) 		0.1 ml 0.03 0.167
Bovine serum
(sterile; heat inactivated at 56°C for 45 min) 		0.7 ml - -
NaHCO3
(10% w/v; sterile) 		0.6 ml 0.06 0.714
Air 5.0 ml - -

Metadata

Last modified: 02.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Hungate tubes, Serum bottle
Description:

Strains can also grow on: Trypticase Soy Agar (BBL 11043) with 5% defibrinated sheep blood or Columbia Agar Plates with 5% sheep blood (BBL 4354005)
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 9
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 0.03 0.357
Glucose 0.015 0.083
Bovine serum - -
Air - -
Trypticase soy broth - -
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Cultivation metadata from 9 strains BacDive

phyla 3 9 strains
oxygen anaerobe 2 strains
temperature mesophilic 9 strains