Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Solution I | 991.0 | ml | - | - | |
Solution II | 8.0 | ml | - | - | |
Solution III | 1.0 | ml | - | - | |
Solution IV | 0.1 | ml | - | - | |
Agar | 15.0 | g/l | - | - | |
1 Final pH of appr. 7.4 should be controlled sterile. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
(NH4)2SO4 | 0.500 | g | 0.505 | 3.818 | |
MgSO4 x 7 H2O | 0.100 | g | 0.101 | 0.409 | |
CaCl2 x 2 H2O | 0.050 | g | 0.05 | 0.343 | |
K2HPO4 | 0.110 | g | 0.111 | 0.637 | |
KH2PO4 | 0.085 | g | 0.086 | 0.63 | |
FeCl3 x 6 H2O | 2.000 | mg | 0.002 | 0.007 | |
Na2-EDTA | 3.000 | mg | 0.003 | 0.009 | |
Na-acetate | 0.546 | g | 0.551 | 6.716 | |
Na2S2O3 x 5 H2O | 0.400 | g | 0.404 | 1.626 | |
Distilled water | 991.000 | ml | - | - | |
1 Autoclave for 20 min. at 121°C. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaHCO3 | 2.625 | g | 52.5 | 624.948 | |
Distilled water | 50.000 | ml | - | - | |
1 NaHCO3 2.625 g per 50 ml distilled water | |||||
2 Autoclave for 20 min. at 121°C and add 8 ml/l sterile solution II shortly, before inoculation of bacterial culture. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Standard vitamin solution | 1 | ml | - | - | |
1 H3P-vitamine solution (see medium 428) | |||||
2 Filter sterilize vitamine solution and add 1 ml/l medium. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Inositol | 1 | g | 100 | 555.062 | |
Distilled water | 10 | ml | - | - | |
1 Final pH of appr. 7.4 should be controlled sterile. | |||||
2 10% inositol (1g/10 ml distilled water) | |||||
3 Filter sterilize solution IV and add 0.1 ml/l medium. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Riboflavin | 10.0 | mg | - | - |
Thiamine-HCl x 2 H2O | 50.0 | mg | - | - |
Nicotinic acid | 50.0 | mg | - | - |
Pyridoxine hydrochloride | 50.0 | mg | - | - |
Calcium pantothenate | 50.0 | mg | - | - |
Biotin | 0.1 | mg | - | - |
Folic acid | 0.2 | mg | - | - |
Vitamin B12 | 1.0 | mg | - | - |
Distilled water | 100.0 | ml | - | - |
Last modified: | 16.01.24 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 7.4 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Na-acetate | 0.546 | 6.656 |
(NH4)2SO4 | 0.5 | 3.784 |
NaHCO3 | 0.42 | 5 |
Na2S2O3 x 5 H2O | 0.4 | 1.612 |
K2HPO4 | 0.11 | 0.632 |
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