845
845 .
SPIRILLUM WINOGRADSKYI MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 845 #
ml
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Peptone
(BD Bacto) |
5 | g | 4.985 | - | |
| Disodium succinate | 1 | g | 0.997 | 6.152 | |
| (NH4)2SO4 | 1 | g | 0.997 | 7.545 | |
| MgSO4 x 7 H2O | 1 | g | 0.997 | 4.045 | |
| FeCl3 x 6 H2O | 2 | mg | 0.002 | 0.007 | |
| MnSO4 x H2O | 2 | mg | 0.002 | 0.012 | |
|
Agar
(BD Bacto) |
1 | g | 0.997 | - | |
| Trace element solution SL-11 | 1 | ml | - | - | |
| Seven vitamins solution | 1 | ml | - | - | |
|
Catalase
(2000 U/ml) |
1 | ml | - | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Dissolve ingredients except trace elements, vitamins and catalase, adjust pH to 7.2 - 7.4, bring medium to the boil and cool to room temperature under 100% N2 gas atmosphere. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. To complete the medium add trace elements, vitamins and catalase from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Vitamins and catalase should be sterilized by filtration. After inoculation, inject sterile air into the culture vessel up to about 20% of the total gas volume (i.e., 4 parts N2 and 1 part air). | |||||
Solution occurs in 22
other media (first defined in Medium 722).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA x 2 H2O | 5.2 | g | 5.2 | 15.467 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml. | |||||
Solution occurs in 78
other media (first defined in Medium 503).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Vitamin B12 | 100 | mg | 0.1 | 0.074 |
| p-Aminobenzoic acid | 80 | mg | 0.08 | 0.583 |
| D-(+)-biotin | 20 | mg | 0.02 | 0.082 |
| Nicotinic acid | 200 | mg | 0.2 | 1.625 |
| Calcium pantothenate | 100 | mg | 0.1 | 0.21 |
| Pyridoxine hydrochloride | 300 | mg | 0.3 | 1.459 |
| Thiamine-HCl x 2 H2O | 200 | mg | 0.2 | 0.536 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 13.04.23 |
| Source: | DSMZ |
| Synonyms: | SPIRILLUM D427 MEDIUM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 - 7.4 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 96 % N2
- 4 % O2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Peptone | 4.985 | - |
| (NH4)2SO4 | 0.997 | 7.545 |
| Disodium succinate | 0.997 | 6.152 |
| MgSO4 x 7 H2O | 0.997 | 4.045 |
| Agar | 0.997 | - |
| Show more | ||
