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847. MACROMONAS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.30 g 0.299 2.266
2 0.30 g 0.299 1.215
3 0.15 g 0.15 1.018
4
(OXOID)
0.20 g 0.2 -
5
(BD Bacto)
1.00 g 0.998 -
6 Seven vitamins solution 1.00 ml - -
7 Trace element solution SL-11 1.00 ml - -
8 1.00 g 0.998 4.021
9 0.50 g 0.499 3.079
10 1000.00 ml 996.215 55298.2
Instructions:
  1. Dissolve ingredients except vitamins, trace elements, thiosulfate and succinate. Adjust pH to 7.2 - 7.4 and bring medium to the boil, distribute into culture vessels and autoclave. To complete the medium add vitamins, trace elements, thiosulfate and succinate from stock solutions sterilized by filtration.
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
Solution occurs in 22 other media (first defined in medium 722 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.2 g 5.2 13.97
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 1000.0 ml 998.207 55408.8
Instructions:
  1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.3
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Na2S2O3 x 5 H2O 0.998 4.021
Agar 0.998 -
Na2-succinate 0.499 3.079
(NH4)2SO4 0.299 2.266
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain

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