Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
(NH4)2SO4 | 0.30 | g | 0.3 | 2.27 | |
MgSO4 x 7 H2O | 0.30 | g | 0.3 | 1.217 | |
CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.02 | |
Yeast extract
(OXOID) |
0.20 | g | 0.2 | - | |
Agar
(BD Bacto) |
1.00 | g | 1 | 2.973 | |
Seven vitamins solution | 1.00 | ml | - | - | |
Trace element solution SL-11 | 1.00 | ml | - | - | |
Na2S2O3 x 5 H2O | 1.00 | g | 1 | 4.029 | |
Disodium succinate | 0.50 | g | 0.5 | 3.085 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except vitamins, trace elements, thiosulfate and succinate. Adjust pH to 7.2 - 7.4 and bring medium to the boil, distribute into culture vessels and autoclave. To complete the medium add vitamins, trace elements, thiosulfate and succinate from stock solutions sterilized by filtration. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Vitamin B12 | 100 | mg | 0.1 | 0.074 |
p-Aminobenzoic acid | 80 | mg | 0.08 | 0.583 |
D-(+)-biotin | 20 | mg | 0.02 | 0.082 |
Nicotinic acid | 200 | mg | 0.2 | 1.625 |
Calcium pantothenate | 100 | mg | 0.1 | 0.21 |
Pyridoxine hydrochloride | 300 | mg | 0.3 | 1.459 |
Thiamine-HCl x 2 H2O | 200 | mg | 0.2 | 0.536 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA x 2 H2O | 5.2 | g | 5.2 | 15.467 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml. |
Last modified: | 07.04.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.2 - 7.4 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Na2S2O3 x 5 H2O | 1 | 4.029 |
Agar | 1 | 2.973 |
Disodium succinate | 0.5 | 3.085 |
(NH4)2SO4 | 0.3 | 2.27 |
MgSO4 x 7 H2O | 0.3 | 1.217 |
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