847 .
MACROMONAS MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
7.2 - 7.4
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 0.30 | g | ||
|
|
MgSO4 x 7 H2O | 0.30 | g | ||
|
|
CaCl2 x 2 H2O | 0.15 | g | ||
|
|
Yeast extract
(OXOID) |
0.20 | g | ||
|
|
Agar
(BD Bacto) |
1.00 | g | ||
|
|
Seven vitamins solution | 1.00 | ml | ||
|
|
Trace element solution SL-11 | 1.00 | ml | ||
|
|
Na2S2O3 x 5 H2O | 1.00 | g | ||
|
|
Disodium succinate | 0.50 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients except vitamins, trace elements, thiosulfate and succinate. Adjust pH to 7.2 - 7.4 and bring medium to the boil, distribute into culture vessels and autoclave. To complete the medium add vitamins, trace elements, thiosulfate and succinate from stock solutions sterilized by filtration. | |||||
Seven vitamins solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Vitamin B12 | 100 | mg |
|
|
p-Aminobenzoic acid | 80 | mg |
|
|
D-(+)-biotin | 20 | mg |
|
|
Nicotinic acid | 200 | mg |
|
|
Calcium pantothenate | 100 | mg |
|
|
Pyridoxine hydrochloride | 300 | mg |
|
|
Thiamine-HCl x 2 H2O | 200 | mg |
|
|
Distilled water | 1000 | ml |
Trace element solution SL-11 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA x 2 H2O | 5.2 | g | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml. | |||||