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848. RAE MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 40.00 g 40 222.03
2 10.00 g 10 -
3 10.00 g 10 -
4 1.50 g 1.5 7.138
5 3.38 g 3.38 18.99
6 10.00 ml - -
7
(Absolute )
10.00 ml 7.9 171.484
8 980.00 ml 978.243 54300.7
Instructions:
  1. Final pH ca. 3.8 (pH is not adjusted)
  2. Both liquid and solid media are prepared by autoclaving the medium without the addition of glacial acetic acid and absolute ethanol. Glacial acetic acid and absolute ethanol are sterilised either by filtration (use Teflon filters) or they may be autoclaved in completely closed screw cap glass bottles sealed with a Teflon coated septum. The preparation of solid media employs a double layer system similar to that described for Acetobacter europaeus medium (see medium 631). Substitute the components listed above and prepare a lower, 0.5% agar layer, which is then overlaid with a 1.0% agar layer as described in medium 631. Incubate in closed containers to keep the humidity high (> 90%).

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 3.8
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 4

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 978.243 54300.7
Glucose 40 222.03
Yeast extract 10 -
Peptone 10 -
Ethanol 7.9 171.484
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Cultivation metadata from 4 strains BacDive

halophily moderate 1 strain
phyla 2 4 strains
oxygen aerobe 1 strain
temperature mesophilic 4 strains

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