DSMZ 848 . RAE MEDIUM

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Culture medium recipe

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Selected strain: Glutamicibacter mysorens DSM 12798

This strain uses the standard medium without modifications.

Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 40.00 g 40 222.025
Peptone 10.00 g 10 -
Yeast extract 10.00 g 10 -
Citric acid x H2O 1.50 g 1.5 7.138
Na2HPO4 x 2 H2O 3.38 g 3.38 23.81
Glacial acetic acid 10.00 ml - -
Absolute ethanol 10.00 ml - -
Distilled water 980.00 ml - -
1 Final pH ca. 3.8 (pH is not adjusted)
2 Both liquid and solid media are prepared by autoclaving the medium without the addition of glacial acetic acid and absolute ethanol. Glacial acetic acid and absolute ethanol are sterilised either by filtration (use Teflon filters) or they may be autoclaved in completely closed screw cap glass bottles sealed with a Teflon coated septum. The preparation of solid media employs a double layer system similar to that described for Acetobacter europaeus medium (see medium 631). Substitute the components listed above and prepare a lower, 0.5% agar layer, which is then overlaid with a 1.0% agar layer as described in medium 631. Incubate in closed containers to keep the humidity high (> 90%).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 2 g - -
Peptone 3 g - -
Glucose 5 g - -
Acetic acid 40 ml - -
Ethanol 30 ml - -
Distilled water 930 ml - -
1 The ethanol and acetic acid are sterilized separately by filtration (use filter resistant to these solvents), and added aseptically to the sterile medium. Acetobacter europaeus will not grow on plates containing 1-2% agar, and a double layer system has to be used. A layer of growth medium containing 0.5% agar (about 35 ml) is poured into a plate and allowed to solidify. A thin layer (5-10 ml) of the growth medium containing 1% agar is then poured on top. Carefully streak the plates, which are best incubated in closed containers (e.g. plastic boxes) at 30°C.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 3.8
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 4
Glutamicibacter mysorens DSM 12798

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 40 222.025
Yeast extract 10 -
Peptone 10 -
Na2HPO4 x 2 H2O 3.38 23.81
Citric acid x H2O 1.5 7.138
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Cultivation metadata from 4 strains BacDive

pH 2 - 4 1 strain
phyla 2 4 strains
oxygen aerobe 1 strain
temperature mesophilic 4 strains