DSMZ 853a . FUSIBACTER TUNISIENSIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 1 18.695
K2HPO4 0.30 g 0.3 1.722
KH2PO4 0.30 g 0.3 2.205
MgCl2 x 6 H2O 3.00 g 3 14.756
CaCl2 x 2 H2O 0.10 g 0.1 0.68
KCl 1.00 g 1 13.414
NaCl 70.00 g 70 1197.81
Yeast extract
(OXOID)
1.00 g 1 -
Trypticase peptone
(BD BBL)
1.00 g 1 -
Na-acetate x 3 H2O 0.50 g 0.5 -
Modified Wolin's mineral solution 10.00 ml - -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
Na2S2O3 x 5 H2O 3.16 g 3.16 12.732
L-Cysteine HCl x H2O 0.50 g 0.5 2.847
Na2CO3 1.50 g 1.5 14.153
D-Glucose 3.60 g 3.6 19.982
Na2S x 9 H2O 0.30 g 0.3 1.249
Distilled water 1000.00 ml - -
1 Dissolve ingredients, except thiosulfate, cysteine, carbonate, glucose and sulfide, then sparge medium for 30 - 45 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Add thiosulfate and cysteine, then adjust pH to 7.0 and dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving, add glucose and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 7.2 - 7.4, if necessary.
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 07.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2 - 7.4
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
D-Glucose 3.6 19.982
Na2S2O3 x 5 H2O 3.16 12.732
MgCl2 x 6 H2O 3 14.756
Na2CO3 1.5 14.153
NH4Cl 1 18.695
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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