860
860 .
ANAEROCOLUMNA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Clostridium sp. DSM 19740
* Modification: Replace fructose with 2.5 g/l Na2S2O3 x 5 H2O and adjust pH of complete medium to 7.4.
Growth conditions: anaerobic
35 °C
Main sol. 860 #
ml
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.0 | g | 0.998 | 18.658 | |
| K2HPO4 | 0.3 | g | 0.299 | 1.719 | |
| KH2PO4 | 0.3 | g | 0.299 | 2.2 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.982 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.679 | |
| KCl | 0.1 | g | 0.1 | 1.339 | |
| NaCl | 0.6 | g | 0.599 | 10.246 | |
| Yeast extract | 1.0 | g | 0.998 | - | |
| Trace element solution SL-10 | 1.5 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 1.5 | g | 1.497 | 14.124 | |
| D-Fructose* | 4.0 | g | 3.992 | 22.158 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.499 | 2.841 | |
| Na2S x 9 H2O | 0.3 | g | 0.299 | 1.247 | |
| Na2S2O3 x 5 H2O* | 2.5 | g | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, fructose, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add fructose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.0 - 7.2, if necessary. | |||||
Solution occurs in 266
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 07.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | * 7.4 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2S2O3 x 5 H2O | 2.495 | 10.053 |
| Na2CO3 | 1.497 | 14.124 |
| NH4Cl | 0.998 | 18.658 |
| Yeast extract | 0.998 | - |
| NaCl | 0.599 | 10.246 |
| Show more | ||
