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866. DESULFOHALOTOMACULUM ALKALIPHILUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.2 g 0.198 1.452
2 0.1 g 0.099 0.486
3 0.2 g 0.198 2.651
4 1.0 g 0.988 18.473
5 5.0 g 4.941 34.783
6 5.0 g 4.941 84.54
7 Trace element solution SL-10 1.0 ml - -
8 1.5 g 1.482 -
9
(0.1% w/v)
0.5 ml 4.9e-4 0.002
10 0.5 g 0.494 4.662
11 8.0 g 7.905 94.101
12 Vitamin solution 10.0 ml - -
13 5.0 g 4.941 72.65
14 0.5 g 0.494 2.057
15 1000.0 ml 986.371 54751.8
16 , might need strain adjustment 2.5 g/l - -
Instructions:
  1. Dissolve the ingredients (except carbonate, bicarbonate, vitamins, formate and sulfide,) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate and bicarbonate, adjust pH to 8.8 - 9.0, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add vitamins (sterilized by filtration), formate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.9
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.211 55353.5
NaHCO3 7.905 94.101
NaCl 4.941 84.54
Sodium formate 4.941 72.65
Na2SO4 4.941 34.783
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Cultivation metadata from 2 strains BacDive

halophily moderate 1 strain
halophily not 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 2 2 strains
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