Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
* Modification: Adjust pH of complete medium to 6.5.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Trypticase soy broth
(BD BBL) |
5.5 | g | 5.5 | - | |
Resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
D-Glucose | 2.0 | g | 2 | 11.101 | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve dehydrated TSB medium and resazurin, bring to the boil and cool to room temperature while sparging with 100% N2 gas. Adjust pH to 5.5, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose from a separately sterilized anoxic stock solution. |
Last modified: | 07.04.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | * 6.5 |
Equipment needed: | Autoclave, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Trypticase soy broth | 5.5 | - |
D-Glucose | 2 | 11.101 |
Resazurin | 5.0e-4 | 0.002 |
Distilled water | - | - |