869
869 .
ANAEROBIC TRYPTIC SOY BROTH MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Clostridium akagii DSM 12554
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
20 °C
Main sol. 869 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase soy broth
(BD BBL) |
5.5 | g | 5.5 | - | |
|
Resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| D-Glucose | 2.0 | g | 2 | 11.101 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve dehydrated TSB medium and resazurin, bring to the boil and cool to room temperature while sparging with 100% N2 gas. Adjust pH to 5.5, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose from a separately sterilized anoxic stock solution. | |||||
Metadata
| Last modified: | 07.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 5.5 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 3 |
| Strains with modifications: | 1 |
Clostridium akagii DSM 12554
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Trypticase soy broth | 5.5 | - |
| D-Glucose | 2 | 11.101 |
| Resazurin | 5.0e-4 | 0.002 |
| Distilled water | - | - |
