DSMZ 869 . ANAEROBIC TRYPTIC SOY BROTH MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Selected strain: Clostridium acidisoli DSM 12555

This strain uses the standard medium without modifications.

Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase soy broth
(BD BBL)
5.5 g 5.5 -
Resazurin
(0.1% w/v)
0.5 ml 5.0e-4 0.002
D-Glucose 2.0 g 2 11.101
Distilled water 1000.0 ml - -
1 Dissolve dehydrated TSB medium and resazurin, bring to the boil and cool to room temperature while sparging with 100% N2 gas. Adjust pH to 5.5, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose from a separately sterilized anoxic stock solution.

Metadata

Last modified: 07.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 5.5
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3
Strains with modifications: 1
Clostridium acidisoli DSM 12555

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Trypticase soy broth 5.5 -
D-Glucose 2 11.101
Resazurin 5.0e-4 0.002
Distilled water - -

Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen anaerobe 3 strains
temperature psychrophilic 2 strains
temperature mesophilic 1 strain