869 .
ANAEROBIC TRYPTIC SOY BROTH MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase soy broth
(BD BBL) |
5.5 | g | ||
|
|
Resazurin
(0.1% w/v) |
0.5 | ml | ||
|
|
D-Glucose | 2.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve dehydrated TSB medium and resazurin, bring to the boil and cool to room temperature while sparging with 100% N2 gas. Adjust pH to 5.5, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose from a separately sterilized anoxic stock solution. | |||||