MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 87 . CHLOROFLEXUS MEDIUM (modified)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 87 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1.00 g 0.947 -
Glycyl-glycine 1.00 g 0.947 7.168
Na2HPO4 x 2 H2O 0.10 g 0.095 0.667
MgSO4 x 7 H2O 0.10 g 0.095 0.384
KNO3 0.10 g 0.095 0.937
NaNO3 0.50 g 0.473 5.571
NaCl 0.10 g 0.095 1.62
CaCl2 x 2 H2O 0.05 g 0.047 0.322
Fe(III) citrate
(0.1 g in 100 ml H2O) 		5.00 ml - -
Trace element solution SL-6 1.00 ml - -
Distilled water 1050.00 ml - -
1 Adjust pH to 8.2.
2 Boil the medium under a stream of nitrogen gas for a few minutes and distribute 10 ml medium into 15 ml hungate tubes. Bubble each tube with nitrogen gas and close immediately with a rubber septum and screw tight.
3 Autoclave at 121 °C for 15 min.
4 After autoclaving inject 0.1 ml of neutralized sulfide solution (0.03% end concentration) to each tube.

Neutralized sulfide solution 3% (w/v) #

100
Solution occurs in 19 other media (first defined in Medium 28).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 3 g 30 124.905
Distilled water 100 ml - -
1 The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121°C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 08.05.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.2
Equipment needed: Autoclave
Edit in Medium builder

Associated strains

All strains for this medium: 4

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glycyl-glycine 0.947 7.168
Yeast extract 0.947 -
NaNO3 0.473 5.571
NaCl 0.095 1.62
KNO3 0.095 0.937
Show more
CSV
JSON

Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
oxygen anaerobe 4 strains
temperature thermophilic 4 strains