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874. FERROPLASMA ACIDIPHILUM MEDIUM

Culture medium recipe

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Go back to medium without modifications
* Modified for Ferroplasma cupricumulans DSM 16651
beta

Supplement medium prior to inoculation with 2.0 g/l potassium tetrathionate added from a stock solution sterilized by filtration. Incubate without agitation.

Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 960 ml - -
2 Solution B 50 ml - -
3 Solution C 10 ml - -
5 , might need strain adjustment x g/l - -
* 2 g/l - -
Instructions:
  1. Add solution B to solution A, adjust pH of the combined soultion to 1.6 - 1.8 with H2SO4, then sterilize using membrane filter and dispense in sterile culture vessels. Prior to use, add the appropriate volume of solution C sterilized by autoclaving.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 0.417 1.691
2 0.2 g 0.208 1.577
3 0.1 g 0.104 1.397
4 0.1 g 0.104 0.598
5 Trace element solution 10.0 ml - -
6 950.0 ml 987.809 54831.7
7 Trace element solution SL-10 , might need strain adjustment 1.0 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 25 g 500 1798.46
2
(1 N )
40 ml - -
3 10 ml 199.641 11081.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(OXOID)
0.2 g 20 -
2 10.0 ml 998.207 55408.8
Solution occurs in 1 other media (first defined in medium 158 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.93 g 1.93 7.14
2 0.18 g 0.18 0.91
3 0.45 g 0.45 1.18
4 22.00 mg 0.022 0.077
5 5.00 mg 0.005 0.029
6 3.00 mg 0.003 0.012
7 3.80 mg 0.004 0.015
8 2.00 mg 0.002 0.007
9 1000.00 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 1.7
Equipment needed: Filter

Associated strains

All strains for this medium: 4
Strains with modifications: 2
Ferroplasma cupricumulans DSM 16651

  2.0 g/l potassium tetrathionate

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Distilled water 959.062 53236
FeSO4 x 7 H2O 24.51 88.16
Potassium tetrathionate 2 -
MgSO4 x 7 H2O 0.392 1.591
(NH4)2SO4 0.196 1.484
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
temperature mesophilic 2 strains
temperature thermophilic 2 strains

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