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DSMZ

DSMZ 878 . THERMUS 162 MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Thermus aquaticus DSM 625

This strain uses the standard medium without modifications.

Main sol. 878 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1.0 g 1 -
Tryptone 1.0 g 1 14.069
Agar 28.0 g 28 83.252
Nitrilotriacetic acid 100.0 mg 0.1 0.523
CaSO4 x 2 H2O 40.0 mg 0.04 0.232
MgCl2 x 6 H2O 200.0 mg 0.2 0.984
Fe(III) citrate
(0.01 M) 		0.5 ml 0.001 0.005
Trace element solution 0.5 ml - -
Phosphate buffer 100.0 ml - -
Distilled water 900.0 ml - -
1 Adjust pH to 7.2 with NaOH. Autoclave at 121°C for 15 min.
2 Autoclave the phosphate buffer separately and then add to the medium.

Phosphate buffer #

1000
Solution occurs in 3 other media (first defined in Medium 630).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 5.44 g 5.44 39.975
Na2HPO4 x 12 H2O 43.00 g 43 302.904
Distilled water 1000.00 ml - -
1 Adjust pH to 7.2

Trace element solution #

1000
Solution occurs in 1 other media (first defined in Medium 878).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2SO4 0.50 ml - -
MnSO4 x H2O 2.28 g 2.28 13.49
ZnSO4 x 7 H2O 0.50 g 0.5 1.739
H3BO3 0.50 g 0.5 8.085
CuSO4 x 5 H2O 25.00 mg 0.025 0.1
Na2MoO4 x 2 H2O 25.00 mg 0.025 0.103
CoCl2 x 6 H2O 45.00 mg 0.045 0.347
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 32
Strains with modifications: 2
Thermus aquaticus DSM 625

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 28 83.252
Na2HPO4 x 12 H2O 4.3 30.29
Tryptone 1 14.069
Yeast extract 1 -
KH2PO4 0.544 3.998
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Cultivation metadata from 32 strains BacDive

pH 6 - 8 3 strains
pH 10 - 12 2 strains
pH 4 - 6 2 strains
pH 8 - 10 1 strain
phyla 4 32 strains
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