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878. THERMUS 162 MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.0 g 0.999 -
2 1.0 g 0.999 -
3 28.0 g 27.972 -
4 100.0 mg 0.1 0.523
5 40.0 mg 0.04 0.232
6 200.0 mg 0.2 0.983
7 0.5 ml - -
8 Trace element solution 0.5 ml - -
9 Phosphate buffer 100.0 ml - -
10 900.0 ml 897.489 49818.1
Instructions:
  1. Adjust pH to 7.2 with NaOH. Autoclave at 121°C for 15 min.
  2. Autoclave the phosphate buffer separately and then add to the medium.
Solution occurs in 2 other media (first defined in medium 1655 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.44 g 5.44 39.975
2 43.00 g 43 120.065
3 1000.00 ml 998.207 55408.8
Instructions:
  1. Adjust pH to 7.2
Solution occurs in 1 other media (first defined in medium 1655 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.50 ml - -
2 2.28 g 2.28 13.49
3 0.50 g 0.5 1.739
4 0.50 g 0.5 8.086
5 25.00 mg 0.025 0.1
6 25.00 mg 0.025 0.103
7 45.00 mg 0.045 0.189
8 1000.00 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave

Associated strains

All strains for this medium: 32
Strains with modifications: 2
Thermus filiformis DSM 4687

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.709 55381.1
Agar 27.972 -
Na2HPO4 x 12 H2O 4.296 11.995
Tryptone 0.999 -
Yeast extract 0.999 -
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Cultivation metadata from 32 strains BacDive

halophily moderate 1 strain
pH 6 - 8 3 strains
pH 8 - 10 2 strains
pH 4 - 6 2 strains
pH 10 - 14 2 strains
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Similar media

1655. MODIFIED THERMUS MEDIUM 878 99 %
630. MODIFIED THERMUS 162 MEDIUM 84 %
86a. MODIFIED CASTENHOLZ MEDIUM 76 %
J821. TRYPTONE-YEAST EXTRACT (ISP-1) AGAR 71 %
1276. MEDIUM FOR CHELATOCOCCUS BUHARII 70 %

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