Name: SULFOLOBUS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 88 #

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
(NH₄)₂SO₄	1.30	g	1.287	9.741
KH₂PO₄	0.28	g	0.277	2.037
MgSO₄ x 7 H₂O	0.25	g	0.248	1.004
CaCl₂ x 2 H₂O	0.07	g	0.069	0.471
FeCl₃ x 6 H₂O	0.02	g	0.02	0.073
Allen's trace element solution	10.00	ml	-	-
Yeast extract (OXOID)	1.00	g	0.99	-
Distilled water	1000.00	ml	-	-
1 Dissolve ingredients (except yeast extract or other substrates), adjust pH of the salt solution at room temperature to 2.0 using 10 N H₂SO₄ and autoclave. Yeast extract and other organic substrates are sterilized separately by autoclaving of a 10% (w/v) stock solution at neutral pH.

Allen's trace element solution #

1000

Solution occurs in 14 other media (first defined in Medium 88).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
MnCl₂ x 4 H₂O	180	mg	0.18	0.91
Na₂B₄O₇ x 10 H₂O	450	mg	0.45	-
ZnSO₄ x 7 H₂O	22	mg	0.022	0.077
CuCl₂ x 2 H₂O	5	mg	0.005	0.029
Na₂MoO₄ x 2 H₂O	3	mg	0.003	0.012
VOSO₄ x 2 H₂O	3	mg	0.003	0.015
CoSO₄ x 7 H₂O	1	mg	1.0e-3	0.006
Distilled water	1000	ml	-	-
1 Adjust pH of final solution to 2 with 1 N HCl.

All strains for this medium:	19
Strains with modifications:	18

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88	SULFOLOBUS MEDIUM
a	SULFOLOBUS MEDIUM (ANAEROBIC)
b	V24N MEDIUM
c	ZESTOSPHAERA MEDIUM
291	PYROBACULUM NEUTROPHILUM MEDIUM