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882. LEPTOSPIRILLUM (HH) MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 950 ml - -
2 Solution B 50 ml - -
3 Solution C 1 ml - -
Instructions:
  1. Sterilize solution A and solution C by autoclaving at 121°C for 20 min and solution B by autoclaving at 112°C for 30 min. Prior to use mix solutions A and B and add trace elements solution. The final pH of the medium should be 1.8.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 132 mg 0.139 1.052
2 53 mg 0.056 0.274
3 27 mg 0.028 0.209
4 147 mg 0.155 1.053
5 950 ml 998.207 55408.8
Instructions:
  1. Adjust pH to 1.8 with 10 N H2SO4.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 20 g 400 1438.77
2
(0.25 N)
50 ml - -
Instructions:
  1. The pH of this solution should be 1.2.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace element solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 76 mg 0.076 0.384
2 68 mg 0.068 0.499
3 64 mg 0.064 0.269
4 31 mg 0.031 0.501
5 10 mg 0.01 0.049
6 67 mg 0.067 0.393
7 1000 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 1.8

Associated strains

All strains for this medium: 12
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 948.346 52641.1
FeSO4 x 7 H2O 19.98 71.867
CaCl2 x 2 H2O 0.147 0.999
(NH4)2SO4 0.132 0.998
MgCl2 x 6 H2O 0.053 0.26
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Cultivation metadata from 12 strains BacDive

pH 2 - 4 1 strain
phyla 2 12 strains
oxygen facultative anaerobe 1 strain
temperature mesophilic 12 strains

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