Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Solution A | 950 | ml | - | - | |
Solution B | 50 | ml | - | - | |
Solution C | 1 | ml | - | - | |
1 Sterilize solution A and solution C by autoclaving at 121°C for 20 min and solution B by autoclaving at 112°C for 30 min. Prior to use mix solutions A and B and add trace elements solution. The final pH of the medium should be 2.3. | |||||
2 Note: At the beginning of growth incubate statically without shaking. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
(NH4)2SO4 | 132 | mg | 0.139 | 1.052 | |
MgCl2 x 6 H2O | 53 | mg | 0.056 | 0.274 | |
KH2PO4 | 27 | mg | 0.028 | 0.209 | |
CaCl2 x 2 H2O | 147 | mg | 0.155 | 1.053 | |
Distilled water | 950 | ml | - | - | |
1 Adjust pH to 2.3 with 10 N H2SO4. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
FeSO4 x 7 H2O | 20 | g | 400 | 1438.77 | |
H2SO4
(0.25 N) |
50 | ml | - | - | |
1 The pH of this solution should be 1.2. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
MnCl2 x 4 H2O | 76 | mg | 0.076 | 0.384 |
ZnCl2 | 68 | mg | 0.068 | 0.499 |
CoCl2 x 6 H2O | 64 | mg | 0.064 | 0.493 |
H3BO3 | 31 | mg | 0.031 | 0.501 |
Na2MoO4 | 10 | mg | 0.01 | 0.049 |
CuCl2 x 2 H2O | 67 | mg | 0.067 | 0.393 |
Distilled water | 1000 | ml | - | - |
Last modified: | 25.03.24 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 2.3 |
Equipment needed: | Autoclave |
Edit in Medium builder |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
FeSO4 x 7 H2O | 19.98 | 71.867 |
CaCl2 x 2 H2O | 0.147 | 0.999 |
(NH4)2SO4 | 0.132 | 0.998 |
MgCl2 x 6 H2O | 0.053 | 0.26 |
KH2PO4 | 0.027 | 0.198 |
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pH | 2 - 4 | 1 strain |
phyla | 2 | 13 strains |
oxygen | facultative anaerobe | 1 strain |
temperature | mesophilic | 13 strains |