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DSMZ

DSMZ 882 . LEPTOSPIRILLUM (HH) MEDIUM

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Equipment needed: Autoclave
Final pH: 2.3
Select a strain (optional):

ml
Compound Amount Unit
Solution A 950 ml
Solution B 50 ml
Solution C 1 ml
1 Sterilize solution A and solution C by autoclaving at 121°C for 20 min and solution B by autoclaving at 112°C for 30 min. Prior to use mix solutions A and B and add trace elements solution. The final pH of the medium should be 2.3.
2 Note: At the beginning of growth incubate statically without shaking.

Solution A #

950
Compound Amount Unit
(NH4)2SO4 132 mg
MgCl2 x 6 H2O 53 mg
KH2PO4 27 mg
CaCl2 x 2 H2O 147 mg
Distilled water 950 ml
1 Adjust pH to 2.3 with 10 N H2SO4.

Solution B #

50
Compound Amount Unit
FeSO4 x 7 H2O 20 g
H2SO4
(0.25 N) 		50 ml
1 The pH of this solution should be 1.2.

Solution C #

1
Compound Amount Unit
Trace element solution 1 ml

Trace element solution #

1000
Compound Amount Unit
MnCl2 x 4 H2O 76 mg
ZnCl2 68 mg
CoCl2 x 6 H2O 64 mg
H3BO3 31 mg
Na2MoO4 10 mg
CuCl2 x 2 H2O 67 mg
Distilled water 1000 ml