DSMZ 883 . CALDIVIRGA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trisodium citrate x 2 H2O 2.94 g 2.908 9.888
(NH4)2SO4 1.30 g 1.286 9.731
KH2PO4 0.28 g 0.277 2.035
MgSO4 x 7 H2O 0.25 g 0.247 1.003
CaCl2 x 2 H2O 0.07 g 0.069 0.471
FeCl3 x 6 H2O 0.02 g 0.02 0.073
Allen's trace element solution 10.00 ml - -
Sodium resazurin
(0.1% w/v)
0.50 ml 4.9e-4 0.002
Sulfur
(powdered)
10.00 g 9.891 308.425
Yeast extract
(OXOID)
0.50 g 0.495 -
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.50 g 0.495 2.059
Distilled water 1000.00 ml - -
1 Dissolve ingredients except sulfur, yeast extract, vitamins and sulfide, then adjust the pH to 3.5 with 4 N H2SO4. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials which contain already the appropriate amount of sulfur, only to 30% of their volume to allow for a large headspace. Autoclave at 105°C for 20 min. Prior to inoculation add yeast extract, vitamins (sterilized by filtration) and sulfide from anoxic stock solutions prepared under 100% N2 gas atmosphere. The final pH of the complete medium should be around 4.0.
2 After inoculation, pressurize the cultivation vessels to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
Solution occurs in 14 other media (first defined in Medium 88).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnCl2 x 4 H2O 180 mg 0.18 0.91
Na2B4O7 x 10 H2O 450 mg 0.45 -
ZnSO4 x 7 H2O 22 mg 0.022 0.077
CuCl2 x 2 H2O 5 mg 0.005 0.029
Na2MoO4 x 2 H2O 3 mg 0.003 0.012
VOSO4 x 2 H2O 3 mg 0.003 0.015
CoSO4 x 7 H2O 1 mg 1.0e-3 0.006
Distilled water 1000 ml - -
1 Adjust pH of final solution to 2 with 1 N HCl.
Solution occurs in 299 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 27.10.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 4.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 50 % N2
  • 40 % H2
  • 10 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 9.891 308.425
Trisodium citrate x 2 H2O 2.908 9.888
(NH4)2SO4 1.286 9.731
Na2S x 9 H2O 0.495 2.059
Yeast extract 0.495 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain