88a
88a .
SULFOLOBUS MEDIUM (ANAEROBIC)
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 88a #
ml
Equipment needed: Autoclave
Water bath
Gassing station
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 1.30 | g | 1.3 | 9.838 | |
| KH2PO4 | 0.28 | g | 0.28 | 2.058 | |
| MgSO4 x 7 H2O | 0.25 | g | 0.25 | 1.014 | |
| CaCl2 x 2 H2O | 0.07 | g | 0.07 | 0.476 | |
| FeCl3 x 6 H2O | 0.02 | g | 0.02 | 0.074 | |
| Allen's trace element solution | 10.00 | ml | - | - | |
|
Sulfur
(powder) |
10.00 | g | 10 | 311.818 | |
|
Yeast extract
(OXOID) |
0.50 | g | 0.5 | - | |
| Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except sulfur, yeast extract and sulfide), adjust pH of the salt solution at room temperature to 4.0 using 1 N H2SO4 and sparge medium with 100% N2 gas to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials that contain already the appropriate amount of sulfur. Sterilize medium by heating cultivation vessels in a boiling water bath for 1 - 2 hours on each of 3 successive days. Add yeast extract and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to inoculation check pH and adjust to 4.0, if necessary. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MnCl2 x 4 H2O | 180 | mg | 0.18 | 0.91 | |
| Na2B4O7 x 10 H2O | 450 | mg | 0.45 | - | |
| ZnSO4 x 7 H2O | 22 | mg | 0.022 | 0.077 | |
| CuCl2 x 2 H2O | 5 | mg | 0.005 | 0.029 | |
| Na2MoO4 x 2 H2O | 3 | mg | 0.003 | 0.012 | |
| VOSO4 x 2 H2O | 3 | mg | 0.003 | 0.015 | |
| CoSO4 x 7 H2O | 1 | mg | 1.0e-3 | 0.006 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH of final solution to 2 with 1 N HCl. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 4.0 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes, Water bath |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 10 | 311.818 |
| (NH4)2SO4 | 1.3 | 9.838 |
| Na2S x 9 H2O | 0.5 | 2.082 |
| Yeast extract | 0.5 | - |
| KH2PO4 | 0.28 | 2.058 |
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