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88a. SULFOLOBUS MEDIUM (ANAEROBIC)

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.30 g 1.287 9.741
2 0.28 g 0.277 2.037
3 0.25 g 0.248 1.004
4 0.07 g 0.069 0.471
5 0.02 g 0.02 0.073
6 Allen’s trace element solution 10.00 ml - -
7
(powdered)
10.00 g 9.901 308.769
8
(OXOID)
0.50 g 0.495 -
9 0.50 g 0.495 2.061
10 1000.00 ml 988.324 54860.2
11 , required for pH adjustment
(1 N)
x - -
Instructions:
  1. Dissolve ingredients (except sulfur, yeast extract and sulfide), adjust pH of the salt solution at room temperature to 4.0 using 1 N H2SO4 and sparge medium with 100% N2 gas to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials that contain already the appropriate amount of sulfur. Sterilize medium by heating cultivation vessels in a boiling water bath for 1 - 2 hours on each of 3 successive days. Add yeast extract and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to inoculation check pH and adjust to 4.0, if necessary.
Solution occurs in 14 other media (first defined in medium 88 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 180 mg 0.18 0.91
2 450 mg 0.45 1.18
3 22 mg 0.022 0.077
4 5 mg 0.005 0.029
5 3 mg 0.003 0.012
6 3 mg 0.003 0.015
7 1 mg 1.0e-3 0.004
8 1000 ml 998.207 55408.8
9 , required for pH adjustment
(1 N)
x - -
Instructions:
  1. Adjust pH of final solution to 2 with 1 N HCl.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 4.0
Equipment needed: Hungate tubes

Associated strains

All strains for this medium: 4
Strains with modifications: 4

Gas composition

  • 100 % N2

Light

4 strains need light for growth. Select a strain for more details.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Sulfur 9.901 308.769
(NH4)2SO4 1.287 9.741
Na2S x 9 H2O 0.495 2.061
Yeast extract 0.495 -
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Cultivation metadata from 4 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 4 strains
oxygen anaerobe 4 strains
oxygen obligate anaerobe 1 strain
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