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88b. V24N MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Anaerobic workbench
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Main sol. 88 1000.0 ml - -
2
(powdered)
1.0 g 1 31.186
3 0.2 g 0.2 -
4 2.0 g 2 -
5 0.4 mg 4.0e-4 0.002
6 0.5 g 0.5 2.082
7 , required for pH adjustment
(25% v/v)
x - -
Instructions:
  1. Prepare medium 88 without yeast extract, then add (per litre): #2-6
  2. Adjust the pH to 6.0 with H2SO4 (25% v/v). Prepare the medium under 100% N2 gas phase and distribute anaerobically into rubber stoppered tubes or bottles (presterilized by autoclaving). The medium is sterilized by heating 2 hours at 85 °C on each of two following days.
Solution occurs in 1 other media (first defined in medium 88 )
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.30 g 1.287 9.741
2 0.28 g 0.277 2.037
3 0.25 g 0.248 1.004
4 0.07 g 0.069 0.471
5 0.02 g 0.02 0.073
6 Allen’s trace element solution 10.00 ml - -
8 1000.00 ml 988.324 54860.2
9 , required for pH adjustment
(10 N)
x - -
Instructions:
  1. Dissolve ingredients (except yeast extract or other substrates), adjust pH of the salt solution at room temperature to 2.0 using 10 N H2SO4 and autoclave. Yeast extract and other organic substrates are sterilized separately by autoclaving of a 10%(w/v) stock solution at neutral pH.
Solution occurs in 14 other media (first defined in medium 88 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 180 mg 0.18 0.91
2 450 mg 0.45 1.18
3 22 mg 0.022 0.077
4 5 mg 0.005 0.029
5 3 mg 0.003 0.012
6 3 mg 0.003 0.015
7 1 mg 1.0e-3 0.004
8 1000 ml 998.207 55408.8
9 , required for pH adjustment
(1 N)
x - -
Instructions:
  1. Adjust pH of final solution to 2 with 1 N HCl.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.0
Equipment needed: Anaerobic workbench, Autoclave

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % N2

Light

1 strain needs light for growth. Select a strain for more details.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Starch 2 -
(NH4)2SO4 1.287 9.741
Sulfur 1 31.186
Na2S x 9 H2O 0.5 2.082
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain

Similar media

88a. SULFOLOBUS MEDIUM (ANAEROBIC) 91 %
358. ACIDIANUS MEDIUM (AEROBIC) 87 %
358a. ACIDIANUS MEDIUM (ANAEROBIC) 87 %
291. PYROBACULUM NEUTROPHILUM MEDIUM 87 %
88. SULFOLOBUS MEDIUM 86 %

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