MediaDive DSMZ Digital Diversity
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder SPARQL endpoint Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 88b . V24N MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 88b #

ml
Equipment needed: Autoclave
1 Prepare medium M.88, then add (per litre):
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.30 g 1.3 9.838
KH2PO4 0.28 g 0.28 2.058
MgSO4 x 7 H2O 0.25 g 0.25 1.014
CaCl2 x 2 H2O 0.07 g 0.07 0.476
FeCl3 x 6 H2O 0.02 g 0.02 0.074
Allen's trace element solution 10.00 ml - -
Distilled water 1000.00 ml - -
Sulfur , powdered
(powdered) 		1.00 g 1 31.182
Yeast extract
(Difco, BD 212750) 		0.20 g 0.2 -
Starch , soluble
(soluble) 		2.00 g 2 -
Resazurin 0.40 mg 4.0e-4 0.002
Na2S x 9 H2O 0.50 g 0.5 2.082
2 Adjust the pH to 6.0 with H2SO4 (25% v/v). Prepare the medium under 100% N2 gas phase and distribute anaerobically into rubber stoppered tubes or bottles (presterilized by autoclaving). The medium is sterilized by heating 2 hours at 85 °C on each of two following days.

Allen's trace element solution #

1000
Solution occurs in 14 other media (first defined in Medium 88).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnCl2 x 4 H2O 180 mg 0.18 0.91
Na2B4O7 x 10 H2O 450 mg 0.45 -
ZnSO4 x 7 H2O 22 mg 0.022 0.077
CuCl2 x 2 H2O 5 mg 0.005 0.029
Na2MoO4 x 2 H2O 3 mg 0.003 0.012
VOSO4 x 2 H2O 3 mg 0.003 0.015
CoSO4 x 7 H2O 1 mg 1.0e-3 0.006
Distilled water 1000 ml - -
1 Adjust pH of final solution to 2 with 1 N HCl.

Metadata

Last modified: 10.06.25
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.0
Equipment needed: Autoclave
Edit in Medium builder

Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Starch 2 -
(NH4)2SO4 1.3 9.838
Sulfur 1 31.182
Na2S x 9 H2O 0.5 2.082
KH2PO4 0.28 2.058
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain