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88c. ZESTOSPHAERA MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.30 g 1.287 9.741
2 0.28 g 0.277 2.037
3 0.25 g 0.248 1.004
4 0.07 g 0.069 0.471
5 0.02 g 0.02 0.073
6 Allen’s trace element solution 10.00 ml - -
7 2.00 g 1.98 7.979
8
(OXOID)
1.00 g 0.99 -
9
(Bacto BD)
1.00 g 0.99 -
10
(Difco BD)
0.50 g 0.495 -
11 0.50 g 0.495 2.061
12 1000.00 ml 988.324 54860.2
Instructions:
  1. Dissolve ingredients (except thiosulfate, yeast extract, Tryptone, Casamino acids and sulfide), sparge medium with 80% N2 and 20% CO2 gas mixture to make it anoxic and dispense medium under same gas atmosphere into serum bottels. After autoclaving add thiosulfate, yeast extract, Tryptone, Casamino acids and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to inoculation check pH and adjust to 6.0, if necessary.
Solution occurs in 14 other media (first defined in medium 88 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 180 mg 0.18 0.91
2 450 mg 0.45 1.18
3 22 mg 0.022 0.077
4 5 mg 0.005 0.029
5 3 mg 0.003 0.012
6 3 mg 0.003 0.015
7 1 mg 1.0e-3 0.004
8 1000 ml 998.207 55408.8
9 , required for pH adjustment
(1 N)
x - -
Instructions:
  1. Adjust pH of final solution to 2 with 1 N HCl.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.0

Associated strains

All strains for this medium: 1

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Na2S2O3 x 5 H2O 1.98 7.979
(NH4)2SO4 1.287 9.741
Tryptone peptone 0.99 -
Yeast extract 0.99 -
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