Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
(NH4)2SO4 | 1.30 | g | 1.3 | 9.838 | |
KH2PO4 | 0.28 | g | 0.28 | 2.058 | |
MgSO4 x 7 H2O | 0.25 | g | 0.25 | 1.014 | |
CaCl2 x 2 H2O | 0.07 | g | 0.07 | 0.476 | |
FeCl3 x 6 H2O | 0.02 | g | 0.02 | 0.074 | |
Allen's trace element solution | 10.00 | ml | - | - | |
Na2S2O3 x 5 H2O | 2.00 | g | 2 | 8.058 | |
Yeast extract
(OXOID) |
1.00 | g | 1 | - | |
Tryptone peptone
(Bacto BD) |
1.00 | g | 1 | - | |
Casamino acids
(Difco BD) |
0.50 | g | 0.5 | - | |
Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except thiosulfate, yeast extract, Tryptone, Casamino acids and sulfide), sparge medium with 80% N2 and 20% CO2 gas mixture to make it anoxic and dispense medium under same gas atmosphere into serum bottels. After autoclaving add thiosulfate, yeast extract, Tryptone, Casamino acids and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to inoculation check pH and adjust to 6.0, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MnCl2 x 4 H2O | 180 | mg | 0.18 | 0.91 | |
Na2B4O7 x 10 H2O | 450 | mg | 0.45 | - | |
ZnSO4 x 7 H2O | 22 | mg | 0.022 | 0.077 | |
CuCl2 x 2 H2O | 5 | mg | 0.005 | 0.029 | |
Na2MoO4 x 2 H2O | 3 | mg | 0.003 | 0.012 | |
VOSO4 x 2 H2O | 3 | mg | 0.003 | 0.015 | |
CoSO4 x 7 H2O | 1 | mg | 1.0e-3 | 0.006 | |
Distilled water | 1000 | ml | - | - | |
1 Adjust pH of final solution to 2 with 1 N HCl. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | 6.0 |
Equipment needed: | Autoclave, Gassing station, Serum bottle |
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Na2S2O3 x 5 H2O | 2 | 8.058 |
(NH4)2SO4 | 1.3 | 9.838 |
Tryptone peptone | 1 | - |
Yeast extract | 1 | - |
Na2S x 9 H2O | 0.5 | 2.082 |
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