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DSMZ

DSMZ 890 . VM1 MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 890 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 20 342.231
MgCl2 x 6 H2O 12.60 g 12.6 61.977
NaHCO3 0.16 g 0.16 1.905
K2HPO4 0.20 g 0.2 1.148
NH4Cl 0.30 g 0.3 5.609
Na2SO4 3.24 g 3.24 22.811
CaCl2 x 2 H2O 2.38 g 2.38 16.189
KCl 0.56 g 0.56 7.512
Modified Wolin's mineral solution II 10.00 ml - -
Sulfur
(powdered) 		0.50 g 0.5 15.591
Distilled water 1000.00 ml - -
1 Adjust pH to 7.0 using H2SO4.
2 Prepare the medium under H2 gas and fill 20 ml medium into 100 ml serum bottles and seal with a rubber stopper. Steam medium for 3 hours on each of 3 successive days. Give an atmosphere of H2/CO2/O2 (78/20/2) with an overpressure of two atmospheres. Incubate under shaking at 65°C.
3 For heterotrophic growth add yeast extract 1.0 g, peptone 5.0 g and starch 2.0 g to above medium. Incubate at 65°C in gas phase of 80% N2 and 20% air.

Modified Wolin's mineral solution II #

1000
Solution occurs in 24 other media (first defined in Medium 700).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
MgSO4 x 7 H2O 3.000 g 3 12.172
MnSO4 x H2O 0.500 g 0.5 2.958
NaCl 1.000 g 1 17.112
FeSO4 x 7 H2O 0.100 g 0.1 0.36
CoSO4 x 7 H2O 0.180 g 0.18 1.161
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnSO4 x 7 H2O 0.180 g 0.18 0.626
CuSO4 x 5 H2O 0.010 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.020 g 0.02 0.042
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.025 g 0.025 0.193
Na2SeO3 x 5 H2O 0.300 mg 3.0e-4 0.001
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
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Gas composition

  • 78 % H2
  • 20 % CO2
  • 2 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 12.6 61.977
Na2SO4 3.24 22.811
KCl 0.56 7.512
Sulfur 0.5 15.591
NH4Cl 0.3 5.609
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain