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DSMZ

DSMZ 895 . ANAEROBRANCA GOTTSCHALKII MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Anaerobranca californiensis DSM 14826

* Modification: Omit starch from the medium, increase the concentration of Tryptone to 2.00 g/l and supplement medium with 4.00 g/l Na2S2O3 x 5 H2O added to the medium from a sterile anoxic stock solution prepared under 100% N2 gas.

Growth conditions: anaerobic 60 °C

Main sol. 895 #

ml
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.00 g 0.987 7.471
NH4Cl 0.40 g 0.395 7.382
Na2S2O3 x 5 H2O 0.10 g 0.099 0.398
K2HPO4 0.50 g 0.494 2.834
MgSO4 x 7 H2O 0.10 g 0.099 0.401
CaCl2 x 2 H2O 0.05 g 0.049 0.336
NaCl 10.00 g 9.872 168.92
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) 		2.00 ml 0.002 0.007
Modified Wolin's mineral solution 10.00 ml - -
Yeast extract
(BD Bacto) 		0.25 g 0.247 -
Tryptone*
(BD Bacto) 		2.00 g - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
L-Cysteine HCl x H2O 0.50 g 0.494 2.81
Na2CO3 3.00 g 2.962 27.942
Starch*
(soluble) 		2.00 g 1.974 -
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S2O3 x 5 H2O* 4.00 g - -
Distilled water 1000.00 ml - -
1 Dissolve all ingredients except cysteine, carbonate, starch and vitamins. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, add cysteine and adjust pH to 7.5, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add starch and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 9.3 - 9.5, if necessary.

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 08.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.3 - 9.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3
Strains with modifications: 2
Anaerobranca californiensis DSM 14826

  Tryptone
  Starch
  4 g Na2S2O3 x 5 H2O
  2 g Tryptone

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 3.949 15.91
Na2CO3 2.962 27.942
Tryptone 1.974 -
(NH4)2SO4 0.987 7.471
K2HPO4 0.494 2.834
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Cultivation metadata from 3 strains BacDive

pH 8 - 10 1 strain
pH 10 - 12 1 strain
phyla 1 3 strains
oxygen anaerobe 3 strains
temperature thermophilic 2 strains
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