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898. ANOXYBACILLUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.20 g 0.198 1.452
2 0.10 g 0.099 0.486
3 0.20 g 0.198 2.651
4 1.00 g 0.988 18.473
5 5.00 g 4.941 84.54
6 Trace element solution SL-10 1.00 ml - -
7
(0.1% w/v)
0.50 ml 4.9e-4 0.002
8 2.76 g 2.727 25.732
9 10.00 g 9.881 117.627
10 , might need strain adjustment 0.50 g - -
11 5.00 g 4.941 27.425
12 Vitamin solution 10.00 ml - -
13 0.50 g 0.494 2.057
14 1000.00 ml 986.371 54751.8
15 , might need strain adjustment 2.70 g/l - -
16 , might need strain adjustment 0.65 g/l - -
Instructions:
  1. Dissolve all ingredients except carbonates, yeast extract, glucose, vitamins and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add carbonate and hydrogencarbonate, adjust pH to 9.0 - 9.5, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add yeast extract, glucose, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 9.5 - 9.7.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.6
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 4
Strains with modifications: 4

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.211 55353.5
NaHCO3 9.881 117.627
NaCl 4.941 84.54
D-Glucose 4.941 27.425
Na2CO3 2.727 25.732
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
oxygen anaerobe 4 strains
temperature mesophilic 3 strains
temperature thermophilic 1 strain

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395b. FERVIDICOCCUS MEDIUM 83 %

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