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DSMZ

DSMZ 898a . ANAEROBACILLUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Alkaliphilus serpentinus DSM 100337

* Modification: Replace D-glucose with 5.00 g/l maltose, increase amount of yeast extract to 1.00 g/l and add 2.50 g/l Na2S2O3 x 5 H2O from an anoxic sterile stock solution after autoclaving.

Growth conditions: 3-7 days anaerobic 30 °C

Main sol. 898a #

ml
Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 0.2 1.467
MgCl2 x 6 H2O 0.10 g 0.1 0.491
KCl 0.20 g 0.2 2.677
NH4Cl 1.00 g 0.998 18.658
NaCl 15.00 g 14.97 256.161
Trace element solution SL-10 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Na2CO3 2.76 g 2.754 25.989
NaHCO3 10.00 g 9.98 118.8
Yeast extract* 1.00 g - -
D-Glucose* 5.00 g 4.99 27.698
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.50 g 0.499 2.078
Na2S2O3 x 5 H2O* 2.50 g - -
Maltose* 5.00 g - -
Distilled water 1000.00 ml - -
1 Dissolve all ingredients except carbonates, yeast extract, glucose, vitamins and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add carbonate and hydrogencarbonate, adjust pH to 8.0 - 8.5, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add yeast extract, glucose, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 8.5 - 9.0.

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 306 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 08.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.5 - 9.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 5
Strains with modifications: 4
Alkaliphilus serpentinus DSM 100337

  2.5 g Na2S2O3 x 5 H2O
  1 g Yeast extract
  5 g Maltose
  Yeast extract
  D-Glucose

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
NaCl 14.97 256.161
NaHCO3 9.98 118.8
Maltose 4.99 14.578
Na2CO3 2.754 25.989
Na2S2O3 x 5 H2O 2.495 10.053
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Cultivation metadata from 5 strains BacDive

pH 8 - 10 2 strains
pH 6 - 8 1 strain
pH 10 - 12 1 strain
phyla 1 5 strains
nutrition organoheterotroph 2 strains
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