DSMZ 898a . ANAEROBACILLUS MEDIUM

What is this page?
Equipment needed: Autoclave Filter Gassing station Hungate tubes

Final gas composition: 100 % N2 read more

Final pH: 8.5 - 9.0

Select a strain (optional):

ml
Compound Amount Unit
KH2PO4 0.20 g
MgCl2 x 6 H2O 0.10 g
KCl 0.20 g
NH4Cl 1.00 g
NaCl 15.00 g
Trace element solution SL-10 1.00 ml
Sodium resazurin
(0.1% w/v)
0.50 ml
Na2CO3 2.76 g
NaHCO3 10.00 g
Yeast extract 0.50 g
D-Glucose 5.00 g
Wolin's vitamin solution (10x) 1.00 ml
Na2S x 9 H2O 0.50 g
Distilled water 1000.00 ml
1 Dissolve all ingredients except carbonates, yeast extract, glucose, vitamins and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add carbonate and hydrogencarbonate, adjust pH to 8.0 - 8.5, then dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add yeast extract, glucose, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 8.5 - 9.0.

Trace element solution SL-10 #

1000
Compound Amount Unit
HCl
(25%)
10.0 ml
FeCl2 x 4 H2O 1.5 g
ZnCl2 70.0 mg
MnCl2 x 4 H2O 100.0 mg
H3BO3 6.0 mg
CoCl2 x 6 H2O 190.0 mg
CuCl2 x 2 H2O 2.0 mg
NiCl2 x 6 H2O 24.0 mg
Na2MoO4 x 2 H2O 36.0 mg
Distilled water 990.0 ml
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.