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9. VY/2 AGAR

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 -
2 1.36 g 1.36 9.251
3 0.50 mg 5.0e-4 3.2e-4
4
(Difco)
15.00 g 15 -
5 1000.00 ml 998.207 55408.8
6 , required for pH adjustment x - -
Instructions:
  1. Sterilize vitamin B12 separately by filtration. Prepare and store yeast cells as autoclaved stock suspension ( 5 g baker's yeast/ 100 ml distilled water, adjust pH to 6.5 and autoclave). Adjust pH of medium to 7.2 with KOH before, and after autoclaving and cooling to 50°C (use pH-indicator paper).
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Main sol. 1118 1 ml - -
2 x - -
Instructions:
  1. Please see our video tutorial and follow the special instructions: 'Reactivation of Myxobacteria' given with the strain entry of our catalogue. For suspending the freeze-dried cells from ampoules, add about 0.5 - 1.0 ml medium MD1 (per liter: casiton 3.0 g; calciumchloride dihydrate 0.7 g; magnesiumsulphate heptahydrate 2.0 g) to the vial with freeze dried material.
Instructions:
  1. Cultures of myxobacteria delivered as active cultures (growing on agar plates): Always use the rim of the swarm as inoculum for fresh media. If the swarms are creamy, transfer high amounts of cell mass to several spots on fresh VY/2 agar medium. If the swarm adheres to the agar or grows within the agar, cut small agar cubes from the rim of the swarm and place onto a fresh agar plate using an appropriate tool such as a lancet. Make sure that the pieces of swarm colonies grown on the agar are transferred to the agar plate. Attempt to place the inoculum in such a way that the swarms are in contact with the fresh agar plate.
  2. Incubate for up to 3 weeks (in particular Sorangium and Nannocystis strains) at the temperature given for the strain, taking measures against desiccation. If there is no growth after ten days, carefully split up the agar-culture-cubes and squeeze the material to the agar plate and reincubate.
Solution occurs in 2 other media (first defined in medium 1118 )
Equipment needed: Filter Autoclave Ice bath
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.0 g 3 -
2 0.7 g 0.7 4.761
3 2.0 g 2 8.114
5 Trace element solution SL-4 1.0 ml - -
6 1000.0 ml 998.207 55408.8
Instructions:
  1. After autoclaving and cooling, add 0.5 mg /l vitamin B12 from a filter sterilized stock solution. pH should be 7.0 -7.2.
Solution occurs in 2 other media (first defined in medium 1118 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.500 g 0.5 1.723
2 0.200 g 0.2 0.719
3 0.010 g 0.01 0.035
4 0.003 g 0.003 0.015
5 0.030 g 0.03 0.485
6 0.020 g 0.02 0.084
7 0.001 g 1.0e-3 0.006
8 0.002 g 0.002 0.008
9 0.003 g 0.003 0.012
10 1000.000 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Taxonomic range:  Yeast
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Ice bath

Associated strains

All strains for this medium: 2843
Strains with modifications: 9
9 VY/2 AGAR
a VY/2, DILUTED MEDIUM

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Agar 15 -
Baker's yeast 5 -
CaCl2 x 2 H2O 1.36 9.251
Vitamin B12 5.0e-4 3.2e-4

Cultivation metadata from 2843 strains BacDive

halophily not 3 strains
halophily slightly 1 strain
pH 6 - 8 7 strains
pH 8 - 10 6 strains
pH 4 - 6 5 strains
phyla 4 2843 strains
nutrition chemoheterotroph 3 strains
oxygen aerobe 6 strains
temperature mesophilic 2841 strains

Similar media

942. AGAR WITH E. COLI AS FEED 79 %
49. C/10 MEDIUM 77 %
67. CY-AGAR 73 %
J341. VY/2-ASW AGAR 73 %
1462. CAULOBACTER PYE MEDIUM 72 %

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