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900. ACETOBACTERIUM TUNDRAE MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.33 g 0.329 4.418
2 0.52 g 0.519 2.553
3 0.30 g 0.299 2.037
4 0.33 g 0.329 6.157
5 0.33 g 0.329 2.42
6 Trace element solution SL-10 1.00 ml - -
7 1.00 g 0.998 -
8
(0.1% w/v)
0.50 ml 5.0e-4 0.002
9 1.50 g 1.497 14.124
10 5.00 g 4.99 27.698
11 Seven vitamins solution 1.00 ml - -
12 0.30 g 0.299 1.705
13 0.30 g 0.299 1.247
14 1000.00 ml 996.215 55298.2
15 , might need strain adjustment 4.00 g/l - -
Instructions:
  1. Dissolve ingredients except carbonate, fructose, vitamins and reducing agents. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add fructose, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The pH of the complete medium should be at 7.0.
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Hungate tubes

Associated strains

All strains for this medium: 3
Strains with modifications: 3

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.2
D-Fructose 4.99 27.698
Na2CO3 1.497 14.124
Yeast extract 0.998 -
MgCl2 x 6 H2O 0.519 2.553
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen anaerobe 3 strains
temperature psychrophilic 2 strains
temperature mesophilic 1 strain

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503a. SPOROMUSA TMAO3 MEDIUM 80 %
294. SYNTROPHUS HQGÖ1 MEDIUM 80 %

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