904a
904a .
MARINITOGA HYDROGENITOLERANS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 904a #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(SIGMA) |
30.0 | g | 30 | - | |
|
MES
(MERCK) |
4.0 | g | 4 | 20.488 | |
|
Yeast extract
(OXOID) |
1.0 | g | 1 | - | |
|
Tryptone
(BD Bacto) |
1.0 | g | 1 | 14.069 | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| D-Glucose | 2.5 | g | 2.5 | 13.877 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except glucose, sulfide and cysteine, adjust pH to 6.0 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials (to 30% of volume) and autoclave. Add glucose, sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. | |||||
Metadata
| Last modified: | 08.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.0 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 3 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sea Salt | 30 | - |
| MES | 4 | 20.488 |
| D-Glucose | 2.5 | 13.877 |
| Tryptone | 1 | 14.069 |
| Yeast extract | 1 | - |
| Show more | ||
