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DSMZ

DSMZ 908 . MEDIUM FOR CHLORATE RESPIRERS

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 908 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 1000 ml - -
Solution B 10 ml - -
Solution C 10 ml - -
Solution D: Trace element solution SL-10 1 ml - -
Solution E: Vitamins 5 ml - -
1 Autoclave solutions A, B and C separately and anaerobically under 80% N2 + 20% CO2 atmosphere. Combine 1000 ml solution A, 10 ml solution B and 10 ml solution C. Add 5 ml vitamin solution E and 1 ml trace element solution D. The pH should be 7.2 without further adjustment.

Solution A #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.25 g 0.25 4.674
NaH2PO4 0.60 g 0.6 5.001
KCl 0.10 g 0.1 1.341
NaHCO3 2.50 g 2.5 29.759
NaClO3 1.00 g 1 -
Na-acetate 1.36 g 1.36 16.579
Distilled water 1000.00 ml - -

Solution B #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 30 mg 3 24.923
CaCl2 x 2 H2O 10 mg 1 6.802
Distilled water 10 ml - -

Solution C #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2MoO4 25 mg 0.025 0.121
Na2WO4 x 2 H2O 25 mg 0.025 0.076
Distilled water 1000 ml - -

Solution D: Trace element solution SL-10 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%; 7.7 M) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Solution E: Vitamins #

1000
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 50 mg 0.05 0.037
Pantothenic acid 50 mg 0.05 0.228
Riboflavin 50 mg 0.05 0.133
Pyridoxamine hydrochloride 10 mg 0.01 0.049
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Nicotinic acid 25 mg 0.025 0.203
Nicotine amide 25 mg 0.025 0.205
alpha-lipoic acid 50 mg 0.05 0.242
p-Aminobenzoic acid 50 mg 0.05 0.365
Thiamine-HCl x 2 H2O 50 mg 0.05 0.134
Distilled water 1000 ml - -
1 Stir for some hours, filter sterilize the solution.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 3
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2.437 29.005
Na-acetate 1.326 16.158
NaClO3 0.975 -
NaH2PO4 0.585 4.874
NH4Cl 0.244 4.555
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen aerobe 3 strains
oxygen anaerobe 3 strains
temperature mesophilic 3 strains