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DSMZ

DSMZ 909 . TREPONEMA DENTICOLA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Treponema denticola DSM 14222

* Modification: 80% N2 plus 10% CO2 plus 10% H2; anaerobic

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 3-7 days anaerobic 37 °C pH 7.00

Main sol. 909 #

ml
Equipment needed: Autoclave Gassing station Filter Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 1000 ml - -
Solution B 45 ml - -
1 Add 0.45 ml Sol. B to 10 ml Sol. A. Inoculate and pressurize the tubes with H2 to 0.5 bar.

Solution A #

1000
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
Brain heart infusion broth 12.50 g 12.5 -
Trypticase
(BBL 11921) 		10.00 g 10 -
Yeast extract 2.50 g 2.5 -
Na-thioglycolate 0.50 g 0.5 4.382
L-Cysteine HCl 1.00 g 1 6.344
L-Asparagine 0.25 g 0.25 1.892
Glucose 2.00 g 2 11.101
Agar 3.00 g 3 -
Distilled water 1000.00 ml - -
1 Boil for 5 min, cool to 50°C while gassing with 80% N2 + 20% CO2. Distribute to anaerobic tubes under the same atmosphere and autoclave.

Solution B #

45
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine pyrophosphate 6 mg 0.133 0.289
NaHCO3 2 g 44.444 529.056
Distilled water 25 ml - -
Volatile fatty acid solution 20 ml - -
Rabbit serum 20 ml - -
1 Sterilize by filtration, add 20 ml rabbit serum, gas with 80% N2 + 20% CO2.

Volatile fatty acid solution #

102
Compound Amount Unit Conc. [g/L] Conc. [mM]
KOH
(0.1 N) 		100.0 ml - -
Isobutyric acid 0.5 ml - -
DL-2-Methylbutyric acid 0.5 ml - -
Isovaleric acid 0.5 ml - -
Valeric acid 0.5 ml - -

Metadata

Last modified: 31.08.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 10 % CO2
  • 10 % H2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 2
Treponema denticola DSM 14222

  80% N2, 10% CO2, 10% H2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Brain heart infusion broth 11.962 -
Trypticase 9.569 -
Agar 2.871 -
Yeast extract 2.392 -
Glucose 1.914 10.623
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Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains